我们很高兴有来自斯坦福大学Karl Deisseroth博士的AAV-和lv -光遗传学结构。对于这些向量的第一次排序，您必须与戴瑟罗斯博士一起填写MTA表格。欲获取MTA，请联系Karl Deisseroth博士和Charu Ramakrishnan。在网上订购时，请上传一份MTA副本。
Charu Ramakrishnan的联系邮箱:charur@stanford.edu．
Karl Deisseroth博士联系邮箱:deissero@stanford.edu．
AAV-EF1a-DIO-EYFP
AAV-EF1a-DIO-mCherry
AAV-EF1a-DIO-hCHR2 -mCherry (H134R)
AAV-EF1a-DIO-hCHR2 -EYFP (H134R)
3.0 AAV-EF1a-DIO-eNpHR -eyfp
AAV-EF1a-DIO-chETA-EYFP
AAV-CaMKIIa——EYFP
AAV-CaMKIIa——mCherry
-EYFP AAV-CaMKIIa hCHR2 (H134R)
-mCherry AAV-CaMKIIa hCHR2 (H134R)
AAV-CaMKIIa- eNpHR 3.0-EYFP
-EYFP AAV-hSyn hCHR2 (H134R)
-mCherry AAV-hSyn hCHR2 (H134R)
AAV-hSyn- eNpHR 3.0-EYFP
AAV-CaMKIIa- C1V1 (t/t)- ts - EYFP
AAV-CaMKIIa- C1V1 (t/t)- ts - mCherry
AAV-EF1a-DIO- C1V1 (t/t)- ts - EYFP
AAV-EF1a-DIO- C1V1 (t/t)- ts - mCherry
-EYFP AAV-CaMKIIa hCHR2 (C128S / D156A)
-mCherry AAV-CaMKIIa hCHR2 (C128S / D156A)
-EYFP AAV-EF1a-DIO hCHR2 (C128S / D156A)
-mCherry AAV-EF1a-DIO hCHR2 (C128S / D156A)
AAV- CaMKIIa - hCHR2(et/tc)- eyfp
AAV- CaMKIIa - hCHR2(et/tc)- mcherry
-EYFP AAV-CaMKIIa hCHR2 (E123A)
-mCherry AAV-CaMKIIa hCHR2 (E123A)
-EYFP AAV-EF1a-DIO hCHR2 (et / tc)
-mCherry AAV-EF1a-DIO hCHR2 (et / tc)
-EYFP AAV-EF1a-DIO hCHR2 (E123A)
-mCherry AAV-EF1a-DIO hCHR2 (E123A)
AAV-EF1a-DIO- eNpHR 3.0- mCherry
AAV- CaMKIIa- eNpHR 3.0- mCherry
AAV- hSyn- eNpHR 3.0- mCherry
AAV- hSyn- hCHR2(E123A)- eyfp
AAV- hSyn- EYFP
AAV-EF1a-mCherry-IRES-WGA-Cre
AAV-GFAP-DIO-hCHR2 -EYFP (H134R)
AAV-GFAP-DIO-hCHR2 -mCherry (H134R)
AAV- CaMKIIa- eArch3.0- EYFP
AAV-EF1a-DIO- eArch3.0- EYFP
AAV- CaMKIIa- eArchT3.0- EYFP
AAV- hSyn- mCherry
AAV-EF1a-DIO- hCHR2(T159C)- EYFP
AAV-EF1a-DIO- hCHR2(T159C)- mCherry
AAV- hSyn- eArch3.0- EYFP
AAV- hThy-1- eNpHR 3.0- EYFP
AAV- CaMKIIa- C1V1 (E162T)- p2A- EYFP
AAV-CaMKIIa- hCHR2(T159C)- p2A- EYFP
AAV-CaMKIIa- hCHR2(T159C)- p2A- mCherry
AAV-CaMKIIa- hCHR2(et/tc)- p2A- EYFP
AAV-CaMKIIa- hCHR2(et/tc)- p2A- mCherry
AAV-CaMKIIa- C1V1 (E162T)- TS-p2A- EYFP
AAV-CaMKIIa- C1V1 (E162T)- TS-p2A- mCherry
AAV-CaMKIIa- C1V1 (t/t)- TS-p2A- EYFP
AAV-CaMKIIa- C1V1 (t/t)- TS-p2A- mCherry
AAV-EF1a-DIO- hCHR2(et/tc)- p2A- EYFP
AAV-EF1a-DIO- hCHR2(et/tc)- p2A- mCherry
AAV-EF1a-DIO- C1V1(E162T)- TS- p2A- EYFP
AAV-EF1a-DIO- C1V1(E162T)- TS- p2A- mCherry
AAV-EF1a-DIO- C1V1(t/t)- TS- p2A- EYFP
AAV-EF1a-DIO- C1V1(t/t)- TS- p2A- mCherry
pLenti- CaMKIIa- eArchT3.0- EYFP
pLenti- CaMKIIa- eArch3.0- EYFP
pLenti- CaMKIIa- eMac3.0- EYFP
pLenti- CaMKIIa- C1V1(E122T)- ts - EYFP
pLenti- CaMKIIa- C1V1(E162T)- ts - EYFP
pLenti- CaMKIIa- C1V1(E122T/E162T)- ts - EYFP
pLenti- CaMKIIa- hCHR2(C128S/D156A)- EYFP
pLenti- CaMKIIa- hCHR2(T159C)- EYFP-WPRE
pLenti- CaMKIIa- hCHR2(E123T/T159C)- EYFP-WPRE
pLenti- EF1a - hCHR2- EYFP-WPRE(又名pLECYT)
pLenti- CaMKIIa- hCHR2-mCherry-WPRE
pLenti- CaMKIIa- hCHR2- EYFP - wpre
pLenti- CaMKIIa- hCHR2(H134R)- EYFP - wpre
pLenti- hSyn - hCHR2(H134R)- EYFP - wpre
pLenti- CaMKIIa- eNpHR 3.0- EYFP
pLenti- hSyn- eNpHR 3.0- EYFP
我们很高兴能收到麻省理工学院Ed Boyden博士的lv光基因结构。订购这些质粒不需要MTA。
慢病毒的结构
FCK-Halo-GFP
FCK-ChR2-GFP
FCK-Arch-GFP
FCK-Mac-GFP
FCK-ArchT-GFP
请注意，FCK和CaMKIIa的启动器是一样的
我们还提供以下在我们实验室开发的质粒。
pLenti-mPGK-LacZ
pLenti-mPGK-EGFP
pLenti-mPGK-mCherry
pLenti-mPGK-Luc-IRES-EGFP
pLenti-mPGK-AcGFP
pLenti-hCMV-EGFP
pLenti-hCMV-mCherry
pLenti-mPGK-LacZ
pLenti-hSyn - EGFP
pLenti-hSyn——mCherry
pLenti-hSyn——mCherry-IRES-EGFP
pLenti-hSyn——Luc-PGK-GFP
pLenti-FCK - EGFP
pLenti-hGFAP - EGFP